ox8 antibodies  (Boster Bio)

Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

Article Title: Spatially resolved multi-omics reveals the renal cortex-metabolic reprogramming of Shenhua Tablet for intervention on IgA nephropathy.

doi: 10.1016/j.phymed.2025.156742

Figure Lengend Snippet: Fig. 2. SHT protects from renal dysfunction, mesangial cell proliferation and inflammatory responses in IgAN rats. (A) The 24 h Upro, Scr and BUN levels in the experimental groups (n = 8 in each group). (B) Representative images showing PCNA and PAS staining and semi-quantitative analysis of mesangial cell pro liferation in the experimental groups (n = 6 in each group). Scale bars, 20 μm. (C) Serum IL-6, IL-1β and TNF-α levels in the experimental groups (n = 8 in each group). (D) Immunohistochemical images and semi-quantitative analysis of OX8 and CD68 expression in the experimental groups. (n = 6 in each group). All sta tistical data are presented as mean ± SD and analyzed by ordinary one-way ANOVA followed by Tukey’s multiple comparison test. Comparative analysis, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, not significant.

Article Snippet: The corresponding stain reagent was PAS Stain Kit (Solarbio, G1281), whereas the antibodies used included PCNA rabbit polyclonal antibody (1:200 dilution, Proteintech, 10,205–2-AP), Rabbit Anti-ODC1 antibody (1:200 dilution, Bioss, bs1294R), Col4 Polyclonal antibody (1:300 dilution, Bioss, bsm56208R), CD68 rabbit polyclonal antibody (1:200 dilution, Servicebio, GB113109), OX8 antibodies (1:200 dilution, boster, A02236–1), and HRP-conjugated goat anti-rabbit IgG (H + l) (1:200 dilution, ServiceBio, GB23303).

Techniques: Staining, Immunohistochemical staining, Expressing, Comparison

Journal: PLoS Genetics

Article Title: Identification of Clec4b as a novel regulator of bystander activation of auto-reactive T cells and autoimmune disease

doi: 10.1371/journal.pgen.1008788

Figure Lengend Snippet: a) Relative expression of Clec4b in five naïve immune-related tissue; spleen, blood, thymus, bone-marrow and inguinal lymph nodes ( n = 5 for spleen and thymus, remaining tissues n = 3 ). b) Clec4b expression of naïve spleen cells. The samples were pooled from three different spleens. Cells were first negatively selected for TCR, and then positively selected for CD4 + and CD8 + cells, respectively. After CD4/CD8 sorting, the remaining cells were selected for CD11b/c. Neutrophils were enriched from blood using Ficoll-Hypaque centrifugation. c) Non-pooled spleen samples were negatively selected for T cells and positively for CD4. The CD4- fraction is the flow-through cell ( n = 5 ). d) Relative Clec4b expression in three naïve Clec4b E3 or one DA sample, all sorted with a Mo-flow FACS sorter. The FACS plot depicts the gating strategy for the three assessed population. Before CD4 and His24 (CD45R), the population determining gating, the cells were negatively sorted by gating for TCR and CD45RA, eliminating T cells and B cells. The cells were His24 - CD4 + (CD4+ DC), His24 - CD4 - (CD4 - DC) or His24 + CD4 + (pDCs) ( n = 3 for Clec4b E3 and n = 1 for DA). e) Relative Clec4b expression in naïve and oil-primed splenocytes selected for TCR-CD4+ and for day 0, day 1 day 2, day 3, and day 5 after oil injection. n = 5 for all groups. Significance stars depict Mann. Whitney Test * = p<0 . 05 , ** = p<0 . 01 , *** = p<0 . 001 and SEM.

Article Snippet: CD8+ or CD11b/c+ non-T cells were selected by using streptavidin-coated Macs beads coupled to biotin conjugated anti-CD8 antibodies (clone OX8, BD Franklin Lakes, NJ, USA) or CD11b/c+ (clone OX42, Biolegend San Diego, CA, USA).

Techniques: Expressing, Centrifugation, Injection, MANN-WHITNEY

Journal: PLoS Genetics

Article Title: Identification of Clec4b as a novel regulator of bystander activation of auto-reactive T cells and autoimmune disease

doi: 10.1371/journal.pgen.1008788

Figure Lengend Snippet: DA rats were injected intradermally with oil and 4 days later the spleens were used as a source of oil primed T cells. These were CSFE labeled and injected intravenously into naïve DA and Clec4b E3 recipients that had been injected intradermaly with mineral oil the same day. a) Proliferation FACS plot describing CFSE intensity in CD4 versus CD8 T cells harvested from spleens of the recipients day 3 after oil injection. CFSE negative T cells are the recipient endogenous T cells. The CFSE high are the non-proliferating T cells from the donors and the CFSE low are the donor T cells that have been proliferating in the recipient. b) Histogram over the distribution of the three groups from a and how they differ in the expression of both Ki67 and MHCII on their surface. c) Graphs describing how the two donor groups, non-dividing and dividing T cells, differ in the expression of MHCII and Ki67 between DA and Clec4b E3 recipients. d,e) Illustration on how the expression of the two cytokines IFNg and IL17 is distributed between the three groups of differentially proliferating T cells between the Clec4b sufficient and deficient recipient rats. d) Illustrative FACS plot. e) Statistics analysis and graph. n = 4 for all groups, Mann Whitney Test * = p<0 . 05 , ** = p<0 . 01 , *** = p<0 . 001 and SEM.

Article Snippet: CD8+ or CD11b/c+ non-T cells were selected by using streptavidin-coated Macs beads coupled to biotin conjugated anti-CD8 antibodies (clone OX8, BD Franklin Lakes, NJ, USA) or CD11b/c+ (clone OX42, Biolegend San Diego, CA, USA).

Techniques: Injection, Labeling, Expressing, MANN-WHITNEY

Journal: PLoS ONE

Article Title: TCR and IL-7 Signaling Are Altered in the Absence of Functional GTPase of the Immune Associated Nucleotide Binding Protein 5 (GIMAP5)

doi: 10.1371/journal.pone.0151837

Figure Lengend Snippet: (A) Total number of thymocytes from wildtype and Gimap5 sph/sph mice at different ages (in weeks) was counted by trypan blue staining (bar graphs). Flow cytometric analysis of thymocytes following staining with anti-CD4 and anti-CD8 antibodies in wild-type and Gimap5 sph/sph mice. (B) Total number of T, CD4 + and CD8 + T cells in pooled lymph nodes (inguinal, axillary, brachial and superficial cervical) from wildtype and Gimap5 sph/sph mice at different ages (in weeks, from the same groups of mice as in A) were counted by trypan blue staining. The absolute numbers of total T, CD4 + and CD8 + T cells were calculated by factoring the frequency of CD3 + T cells and the frequency of CD4 + and CD8 + cells within the CD3 + gate. (C) The phenotype of CD4 + or CD8 + SP thymocytes from wild-type and Gimap5 sph/sph mice for the indicated markers is shown as histograms. The data for TCR expression shown in the enlarged histogram and the overlap histogram for CD8 + SP are from different mice. (D) The phenotype of CD4 + or CD8 + T cells from wild-type and Gimap5 sph/sph mice for the indicated markers is shown as histograms. Data shown are representative of 3 independent experiments. Each experiment consisted of analyzing 2 individual sex- and age-matched mice from each genotype.

Article Snippet: For the purification of T cells from the rats, briefly, lymph node suspensions were incubated with biotinylated anti-rat anti-CD8 antibody (OX8) and anti-Ig kappa antibody (MARK-1) antibody followed by incubation with anti-biotin beads (catalog # 11047-Dynabeads, Thermoscientific Fisher).

Techniques: Staining, Expressing

Journal: PLoS ONE

Article Title: TCR and IL-7 Signaling Are Altered in the Absence of Functional GTPase of the Immune Associated Nucleotide Binding Protein 5 (GIMAP5)

doi: 10.1371/journal.pone.0151837

Figure Lengend Snippet: (A) CFSE-labeled polyclonal lymphocytes from wildtype and Gimap5 sph/sph mice were cultured with anti-CD3 antibody, anti-CD3&CD28 antibodies, IL-2 (10 ng/ml), IL-7 (5 ng/ml), IL-15 (10 ng/ml) or PMA/Ionomycin for 3 to 5 days. Medium and cytokines were replenished on day 3. The representative histogram shows CFSE dye dilution, indicative of proliferation in the gated CD4 + T cells. Cultures stimulated with IL-2, IL-7 and IL-15 were analyzed on day 5. Unstimulated cultures and cultures stimulated with anti-CD3 or anti-CD3/CD28 antibodies and PMA/inomycin were analyzed on day 3. (B) Flow cytometric analysis of thymocytes and lymph node populations by staining with anti-TCR, anti-CD4 and anti-CD8 antibodies in wild-type and Gimap5 sph/sph OTII TCR transgenic mice. Data shown are representative of 3 four-week old mice. (C) Purified CD4 + T cells from OT-II TCR-transgenic wild type and Gimap5 sph/sph mice were activated with irradiated APCs in the presence of OVA peptide and cellular proliferation was measured by [ 3 H]-thymidine incorporation. Data were pooled from 3 independent mice in each group. *** P value <0.005.

Article Snippet: For the purification of T cells from the rats, briefly, lymph node suspensions were incubated with biotinylated anti-rat anti-CD8 antibody (OX8) and anti-Ig kappa antibody (MARK-1) antibody followed by incubation with anti-biotin beads (catalog # 11047-Dynabeads, Thermoscientific Fisher).

Techniques: Labeling, Cell Culture, Staining, Transgenic Assay, Purification, Irradiation

Journal: PLoS ONE

Article Title: TCR and IL-7 Signaling Are Altered in the Absence of Functional GTPase of the Immune Associated Nucleotide Binding Protein 5 (GIMAP5)

doi: 10.1371/journal.pone.0151837

Figure Lengend Snippet: (A) CD4 + T cells were purified from freshly isolated lymphocytes from wild type and Gimap5 sph/sph mice. Equal amounts of protein lysates were subjected to Western blot analysis for the expression of FOXO1 and pFOXO1. Data shown are representative of 3 independent experiments. (B) Thymocytes and lymph node cells were stained with antibodies to CD4 and CD8. CD127 expression was assessed in gated CD4 + SP or CD8 + SP thymocytes and CD4 + or CD8 + T cell subsets by flow cytometry. Data shown are representative of 3 independent experiments. (C) Purified CD4 + T from Gimap5 sph/sph mice or Gimap5 lyp/lyp rats were stimulated with IL-7 or IL-15 at 37°C. At the indicated time points the cells were lysed and subjected to Western blot analysis for the detection of phosphorylated STAT5, total STAT5 or Bcl-2. Lower panel represents the densitometric data pooled from 3 independent experiments. * p value < 0.05.

Article Snippet: For the purification of T cells from the rats, briefly, lymph node suspensions were incubated with biotinylated anti-rat anti-CD8 antibody (OX8) and anti-Ig kappa antibody (MARK-1) antibody followed by incubation with anti-biotin beads (catalog # 11047-Dynabeads, Thermoscientific Fisher).

Techniques: Purification, Isolation, Western Blot, Expressing, Staining, Flow Cytometry